cell separation media Search Results


density gradient centrifugation  (Cedarlane)
92
Cedarlane density gradient centrifugation
Density Gradient Centrifugation, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
density gradient centrifugation - by Bioz Stars, 2026-06
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blood mononuclear cells pbmcs  (Cedarlane)
95
Cedarlane blood mononuclear cells pbmcs
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
Blood Mononuclear Cells Pbmcs, supplied by Cedarlane, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
blood mononuclear cells pbmcs - by Bioz Stars, 2026-06
95/100 stars
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lympholytem  (Cedarlane)
95
Cedarlane lympholytem
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
Lympholytem, supplied by Cedarlane, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
lympholytem - by Bioz Stars, 2026-06
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lympholyte poly  (Cedarlane)
93
Cedarlane lympholyte poly
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
Lympholyte Poly, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
lympholyte poly - by Bioz Stars, 2026-06
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protocol lympholyte  (Cedarlane)
94
Cedarlane protocol lympholyte
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
Protocol Lympholyte, supplied by Cedarlane, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
protocol lympholyte - by Bioz Stars, 2026-06
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lympholyte rat density separation medium  (Cedarlane)
93
Cedarlane lympholyte rat density separation medium
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
Lympholyte Rat Density Separation Medium, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
lympholyte rat density separation medium - by Bioz Stars, 2026-06
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lympholyte cell separation media  (Cedarlane)
93
Cedarlane lympholyte cell separation media
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
Lympholyte Cell Separation Media, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
lympholyte cell separation media - by Bioz Stars, 2026-06
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1-step polymorphs human cell separation media  (Accurate Chemical & Scientific Corporation)
90
Accurate Chemical & Scientific Corporation 1-step polymorphs human cell separation media
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
1 Step Polymorphs Human Cell Separation Media, supplied by Accurate Chemical & Scientific Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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lymphocyte-h cell separation media  (Merck KGaA)
90
Merck KGaA lymphocyte-h cell separation media
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
Lymphocyte H Cell Separation Media, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
lymphocyte-h cell separation media - by Bioz Stars, 2026-06
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cell separation media  (Merck KGaA)
90
Merck KGaA cell separation media
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
Cell Separation Media, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cell separation media - by Bioz Stars, 2026-06
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lympholyte cell separation media  (HAOYANG LTD)
90
HAOYANG LTD lympholyte cell separation media
Flow analysis of circulating Tfh cells in GD patients. Human <t>PBMCs</t> from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.
Lympholyte Cell Separation Media, supplied by HAOYANG LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
lympholyte cell separation media - by Bioz Stars, 2026-06
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Image Search Results


Flow analysis of circulating Tfh cells in GD patients. Human PBMCs from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.

Journal: Journal of Immunology Research

Article Title: Constitutive Changes in Circulating Follicular Helper T Cells and Their Subsets in Patients with Graves' Disease

doi: 10.1155/2018/8972572

Figure Lengend Snippet: Flow analysis of circulating Tfh cells in GD patients. Human PBMCs from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4 + CXCR5 + CD45RA − Tfh (cTfh) cells; (c) the numbers of CD4 + CXCR5 + CD45RA − ICOS + T cells; (d) CD4 + CXCR5 + CD45RA − PD-1 + Tfh cells; (e) the correlation between PD-1 + Tfh cell proportions and TPO-Ab levels in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.

Article Snippet: The peripheral blood mononuclear cells (PBMCs) were separated immediately by density gradient separation with Ficoll-Hypaque solution (CL5020, CEDARLANE, Canada).

Techniques: Staining, Flow Cytometry

Expanded frequency of circulating plasma cells in GD patients. Human PBMCs from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD19, anti-CD38, and anti-CD27. (a) The cells were gated initially on lymphocytes and then on CD19 + B cells; afterwards, the numbers of CD27 high CD38 high PCs were analyzed by flow cytometry. (b) Changes in PCs in GD patients. (c) Relation of PC proportions and serum TPO-Ab levels in GD patients. (d) Relation of Tfh2 subset proportions with PCs in GD patients. (e) The correlation between the proportions of PCs and ICOS + Tfh cells in GD patients. (f) The correlation between the proportions of PCs and PD-1 + Tfh cells in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.

Journal: Journal of Immunology Research

Article Title: Constitutive Changes in Circulating Follicular Helper T Cells and Their Subsets in Patients with Graves' Disease

doi: 10.1155/2018/8972572

Figure Lengend Snippet: Expanded frequency of circulating plasma cells in GD patients. Human PBMCs from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD19, anti-CD38, and anti-CD27. (a) The cells were gated initially on lymphocytes and then on CD19 + B cells; afterwards, the numbers of CD27 high CD38 high PCs were analyzed by flow cytometry. (b) Changes in PCs in GD patients. (c) Relation of PC proportions and serum TPO-Ab levels in GD patients. (d) Relation of Tfh2 subset proportions with PCs in GD patients. (e) The correlation between the proportions of PCs and ICOS + Tfh cells in GD patients. (f) The correlation between the proportions of PCs and PD-1 + Tfh cells in GD patients. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns: no significant difference.

Article Snippet: The peripheral blood mononuclear cells (PBMCs) were separated immediately by density gradient separation with Ficoll-Hypaque solution (CL5020, CEDARLANE, Canada).

Techniques: Clinical Proteomics, Staining, Flow Cytometry